Endothelial cells cultured in transwell plates were removed from normal growth conditions and incubated at RT for 20 min prior to evaluation of TEER. Barrier integrity was evaluated using an EndOhm chamber with an EVOM-2 voltmeter (World Precision Instruments, Sarasota, FL, United States) to measure resistance, and the TEER was calculated using the following equation:
where TEERREPORTED is the calculated resistance of the barrier in Ω × cm2, RTOTAL is the resistance measured across a Matrigel-coated insert seeded with HUVECs, RBLANK is the resistance measured across a Matrigel-coated insert without cells, and MAREA is the growth area (0.33 cm2) of the transwell insert (Srinivasan et al., 2015 (link)). Reported TEER values ≥ 12 Ω × cm2 were indicative of an intact endothelial barrier (Dewi et al., 2004 (link)).
where TEERREPORTED is the calculated resistance of the barrier in Ω × cm2, RTOTAL is the resistance measured across a Matrigel-coated insert seeded with HUVECs, RBLANK is the resistance measured across a Matrigel-coated insert without cells, and MAREA is the growth area (0.33 cm2) of the transwell insert (Srinivasan et al., 2015 (link)). Reported TEER values ≥ 12 Ω × cm2 were indicative of an intact endothelial barrier (Dewi et al., 2004 (link)).
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