Two human ovarian cancer cell lines, Hey and SKOV3; and patient-derived xenograft (PDX) cell line (MDACC-HGSC-1, derived from human HGSC) were cultured in a corresponding medium as described previously [6 (link), 7 (link)]. The origin and purity of all cell lines were verified with short tandem repeat (STR) analysis before their use in MD Anderson’s Characterized Cell Line Core (https://www.mdanderson.org/education-and-research/resources-for-professionals/scientific-resources/core-facilities-and-services/characterized-cell-line-core-facility/index.html ).
Paclitaxel (500 nM, Sigma), recombinant human IL-6 protein (R&D Systems, Minneapolis, MN, USA), IL-6R alpha antibody (R&D Systems, for in vitro use only), IL-6R alpha antibody (tocilizumab, for in vivo use in PDX models; Genentech, San Francisco, CA, USA), and apigenin (Sigma) were used in test groups as described in detail below.
Paclitaxel (500 nM, Sigma), recombinant human IL-6 protein (R&D Systems, Minneapolis, MN, USA), IL-6R alpha antibody (R&D Systems, for in vitro use only), IL-6R alpha antibody (tocilizumab, for in vivo use in PDX models; Genentech, San Francisco, CA, USA), and apigenin (Sigma) were used in test groups as described in detail below.
Free full text: Click here
