Male and female donors 18–68 years of age with no known history of neuropsychiatric or neurological conditions (‘control’ cases) were considered for inclusion in this study (Extended Data Table 1). Routine serological screening for infectious disease (HIV, hepatitis B and hepatitis C) was conducted using donor blood samples, and only those donors who were negative for all three tests were considered for inclusion. Only those specimens with RNA integrity (RIN) values of 7.0 or more were considered for inclusion. Postmortem brain specimens were processed as described3 (link). Briefly, coronal brain slabs were cut at 1 cm intervals and frozen for storage at −80 °C until further use. Putative hand and trunk-lower limb regions of the primary motor cortex were identified, removed from slabs of interest, and subdivided into smaller blocks. One block from each donor was processed for cryosectioning and fluorescent Nissl staining (Neurotrace 500/525, ThermoFisher Scientific). Stained sections were screened for histological hallmarks of primary motor cortex. After verifying that regions of interest contained M1, blocks were processed for nucleus isolation as described below.
Bakken T.E., Jorstad N.L., Hu Q., Lake B.B., Tian W., Kalmbach B.E., Crow M., Hodge R.D., Krienen F.M., Sorensen S.A., Eggermont J., Yao Z., Aevermann B.D., Aldridge A.I., Bartlett A., Bertagnolli D., Casper T., Castanon R.G., Crichton K., Daigle T.L., Dalley R., Dee N., Dembrow N., Diep D., Ding S.L., Dong W., Fang R., Fischer S., Goldman M., Goldy J., Graybuck L.T., Herb B.R., Hou X., Kancherla J., Kroll M., Lathia K., van Lew B., Li Y.E., Liu C.S., Liu H., Lucero J.D., Mahurkar A., McMillen D., Miller J.A., Moussa M., Nery J.R., Nicovich P.R., Niu S.Y., Orvis J., Osteen J.K., Owen S., Palmer C.R., Pham T., Plongthongkum N., Poirion O., Reed N.M., Rimorin C., Rivkin A., Romanow W.J., Sedeño-Cortés A.E., Siletti K., Somasundaram S., Sulc J., Tieu M., Torkelson A., Tung H., Wang X., Xie F., Yanny A.M., Zhang R., Ament S.A., Behrens M.M., Bravo H.C., Chun J., Dobin A., Gillis J., Hertzano R., Hof P.R., Höllt T., Horwitz G.D., Keene C.D., Kharchenko P.V., Ko A.L., Lelieveldt B.P., Luo C., Mukamel E.A., Pinto-Duarte A., Preissl S., Regev A., Ren B., Scheuermann R.H., Smith K., Spain W.J., White O.R., Koch C., Hawrylycz M., Tasic B., Macosko E.Z., McCarroll S.A., Ting J.T., Zeng H., Zhang K., Feng G., Ecker J.R., Linnarsson S, & Lein E.S. (2021). Comparative cellular analysis of motor cortex in human, marmoset and mouse. Nature, 598(7879), 111-119.
Brain Donor Donor bloodDonors Female FrozenHepatitis bHepatitis c Infectious diseaseIsolation Lower limb Male Neurological conditions Nucleus Postmortem Primary motor cortex Regions blocks
Corresponding Organization : Allen Institute for Brain Science
Other organizations :
Harvard University, University of California, San Diego, Salk Institute for Biological Studies, Cold Spring Harbor Laboratory, Leiden University Medical Center, J. Craig Venter Institute, University of Washington, University of Maryland, Baltimore, University of Maryland, College Park, Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute, University of Connecticut, Karolinska Institutet, James S. McDonnell Foundation, Icahn School of Medicine at Mount Sinai, Delft University of Technology, Neurological Surgery, University of California, Los Angeles, Broad Institute, Ludwig Cancer Research, McGovern Institute for Brain Research, Howard Hughes Medical Institute
Male and female donors 18–68 years of age with no known history of neuropsychiatric or neurological conditions
Negative for HIV, hepatitis B, and hepatitis C
controls
Positive control: None mentioned
Negative control: None mentioned
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