Pluripotent stem cell lines from HD patients (iPSHD11, iPSHD22, iPSHD34) and healthy controls (iPSRG2L, endo-iPS12, hESM01) were cultured in mTeSR1 medium (Stemcell Technologies, Canada) on a Matrigel™ substrate (BD Biosciences, USA) as described by the manufacturer. Striatal neurons were differentiated and characterized as described previously[10 (link)]. Study was approved by local ethics committee.
Protocol detail
Induced Pluripotent Stem Cell Lines for Huntington's Disease Modeling
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Cell lines
Cultured medium
Endo
Local ethics committee
Matrigel
Neurons
Patients
Stem
Stemcell
Striatal
Corresponding Organization :
Other organizations : Vavilov Institute of General Genetics
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Variable analysis
independent variables
- Pluripotent stem cell lines from HD patients (iPSHD11, iPSHD22, iPSHD34)
- Pluripotent stem cell lines from healthy controls (iPSRG2L, endo-iPS12, hESM01)
- Striatal neurons differentiated from the pluripotent stem cell lines
- Culture conditions (mTeSR1 medium, Matrigel™ substrate)
- Differentiation protocol for striatal neurons
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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