Preparation of Single-Cell Suspensions

To prepare single-cell suspensions, lungs were minced, digested with collagenase and DNase for 30 min at 37 °C, and passed through a 40-μm cell strainer prior to erythrocyte lysis. Single-cell suspensions of bone marrow were prepared as described above, and mouse blood was collected in EDTA tubes, subjected to erythrocyte lysis, and passed through a 40-μm cell strainer. Cells were stained with myeloid or bone marrow antibody panels (Supplementary Table S1) as described previously^{65 (link),68 (link)}. Analyses were carried out on a BD 5-laser LSR II (BD Biosciences) at the Vanderbilt Flow Cytometry Shared Resource, and analyses were performed using FlowJo software (Treestar Inc.).