The protocol and procedure for western blotting were as described in our previous reports 30 (link), 44 (link), 45 (link). The anti-p-YAP (Ser127), anti-YAP, anti-RalA and anti-β-actin primary antibodies were obtained from Cell Signalling Technology (Danvers, MA, USA). The anti-Wnt5a, anti-Wnt5b, anti-Aggrecan and anti-SOX9 primary antibodies were obtained from Abcam (Cambridge, MA, USA). The anti-CD63, anti-CD9, anti-CD81 and anti-Alix were obtained from System Biosciences (Palo Alto, CA, USA). Based on previous research 46 (link), 47 (link), collagen type II was analysed using 5% (wt/vol) SDS-PAGE with anti-collagen type II primary antibodies (Chemicon®, Merck-Millipore).
Protocol detail
Western Blotting Protocol for Protein Analysis
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Corresponding Organization : Shanghai Jiao Tong University
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Variable analysis
independent variables
- Anti-p-YAP (Ser127) primary antibody
- Anti-YAP primary antibody
- Anti-RalA primary antibody
- Anti-β-actin primary antibody
- Anti-Wnt5a primary antibody
- Anti-Wnt5b primary antibody
- Anti-Aggrecan primary antibody
- Anti-SOX9 primary antibody
- Anti-CD63 primary antibody
- Anti-CD9 primary antibody
- Anti-CD81 primary antibody
- Anti-Alix primary antibody
- Anti-collagen type II primary antibody
- P-YAP (Ser127) protein expression
- YAP protein expression
- RalA protein expression
- β-actin protein expression
- Wnt5a protein expression
- Wnt5b protein expression
- Aggrecan protein expression
- SOX9 protein expression
- CD63 protein expression
- CD9 protein expression
- CD81 protein expression
- Alix protein expression
- Collagen type II protein expression
- Western blotting protocol and procedure as described in previous reports 30, 44, 45
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