We used the following antibodies for immunostaining: hamster anti-CD31, rabbit anti-vone Willebrand Factor (vWF) (both from Dako), goat anti-BMP4, goat anti-MGP, rabbit anti-VEGF, rabbit anti-VE-Cadherin (all from Santa Cruz Biotechnology), mouse anti-Perilipin (Cell Signaling Technology).
Immunostaining of Cellular Markers
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Corresponding Organization : University of California, Los Angeles
Variable analysis
- Antibodies used for immunostaining:
- - hamster anti-CD31
- - rabbit anti-von Willebrand Factor (vWF)
- - goat anti-BMP4
- - goat anti-MGP
- - rabbit anti-VEGF
- - rabbit anti-VE-Cadherin
- - mouse anti-Perilipin
- Staining patterns of the various proteins observed using fluorescence microscopy
- Cell culture conditions (e.g., growth in chamber slides)
- Fixation in 4% paraformaldehyde
- Permeabilization with 0.2% Triton X-100
- Blocking with 10% goat serum and 1% BSA in PBS
- Incubation with primary antibodies or non-specific IgG control antibodies
- Incubation with secondary antibodies conjugated with fluorescent dyes (AF-488 or AF-594)
- Nuclear staining with DAPI
- Positive control: Non-specific IgG control antibodies
- Negative control: No staining observed with non-specific IgG control antibodies
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