The wild-type (WT) and mutant (mut) HOXB1 vectors were constructed in pmirGLO vector 3`-UTR. The oligonucleotide sequences used for luciferase analysis were as follows: HOXB1-WT 5‘-CCUGGCAUAUUUAUAUUGCACUA-3ʹ; In DLD-1 cell line, 3 ‘UTR-WT or 3ʹ UTR-mut transfection HOXB1-mut 5ʹ-CCUGGCAUAU UUAUAUCATCAGA-3 ‘, miR-NC or miR-mimics, each were 20 nM. Cell luciferase activity was assessed 48 h later by the dual-luciferase reporting system (Promega, Madison, USA). The experiment was carried out according to the method mentioned before [26 (link)].
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