Western blotting was carried out as previously described.5 (link) Connexin32 Ab was purchased from Santa Cruz Biotechnology. The GAPDH Ab was obtained from Ray Antibody Biotech. β‐Tubulin Ab and HRP‐conjugated secondary Abs were acquired from Sigma‐Aldrich. The RIP1, RIP3, MLKL, p‐RIP1, p‐MLKL, and Src Abs were obtained from Cell Signaling Technology. Phosphor‐caspase 8 (Tyr380) primary Ab was from Abcam.
Protocol detail
Western Blot Analysis of Cell Signaling Proteins
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Xinjiang Medical University
Other organizations : Guangzhou Medical University
Top 5 similar protocols
Variable analysis
independent variables
- Connexin32 Ab
- GAPDH Ab
- β‐Tubulin Ab
- HRP‐conjugated secondary Abs
- RIP1 Ab
- RIP3 Ab
- MLKL Ab
- P‐RIP1 Ab
- P‐MLKL Ab
- Src Ab
- Phosphor‐caspase 8 (Tyr380) primary Ab
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!