The present work did not include any materials obtained directly from human participants and only used MHCC97-H cells purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The usage of the cell lines was permitted by the ethics committee of the Fifth Medical Center, General Hospital of the Chinese PLA (previously named the Beijing 302 Hospital). All experiments were performed according to the Declaration of Helsinki (World Health Organization).45 (link)
For proliferation analysis, cells were seeded in 96-well plates (5 × 103 cells per well) (Corning, NY, USA). Cells were cultured in DMEM (complete Dulbecco’s modified Eagle’s medium, Invitrogen, USA) with 10% FBS (fetal bovine serum, Invitrogen, USA) at 37°C with 5% CO2 for 24 h.41 (link) Treat the MHCC97-H cells with indicated concentration (10.0 μmol/L, 3.0 μmol/L, 1.0 μmol/L, 0.3 μmol/L, 0.1 μmol/L, 0.03 μmol/L or 0.01 μmol/L) of molecular targeting agents for 48 h in MTT experiments.49 (link),50 (link) The relative survival-cell number was reflected by OD 490 nm and the inhibitory rates of molecular targeting agents on MHCC97-H cells were calculated as (control group’s OD 490 nm – administration group’s OD 490 nm)/(control group’s OD 490 nm) × 100%. The IC50 values of molecular targeting agents on MHCC97-H cells were calculated by inhibitory rates.51 (link),52 (link)
For proliferation analysis, cells were seeded in 96-well plates (5 × 103 cells per well) (Corning, NY, USA). Cells were cultured in DMEM (complete Dulbecco’s modified Eagle’s medium, Invitrogen, USA) with 10% FBS (fetal bovine serum, Invitrogen, USA) at 37°C with 5% CO2 for 24 h.41 (link) Treat the MHCC97-H cells with indicated concentration (10.0 μmol/L, 3.0 μmol/L, 1.0 μmol/L, 0.3 μmol/L, 0.1 μmol/L, 0.03 μmol/L or 0.01 μmol/L) of molecular targeting agents for 48 h in MTT experiments.49 (link),50 (link) The relative survival-cell number was reflected by OD 490 nm and the inhibitory rates of molecular targeting agents on MHCC97-H cells were calculated as (control group’s OD 490 nm – administration group’s OD 490 nm)/(control group’s OD 490 nm) × 100%. The IC50 values of molecular targeting agents on MHCC97-H cells were calculated by inhibitory rates.51 (link),52 (link)
