Western Blot Analysis of DNA Repair Proteins

Cell extracts were prepared and proteins were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis as described previously (47 (link),54 (link)). Rabbit anti-53BP1 were purchased from Novus and used at 1:1000 dilution. Mouse anti-BRCA1 (D-9, Santa Cruz) was used at 1:100 for western blotting. HA.11 clone 16B12 from Covance was used at 1:1000 for western blotting. Mre11 was used at 1:1000 for western blotting (Novus Biologicals). Secondary antibodies used were goat-anti-mouse IgG–horseradish peroxidase (HRP) conjugated, goat-anti-rabbit IgG–HRP conjugated at 1:2000 dilutions.

Free full text: Click here

Xiong X., Du Z., Wang Y., Feng Z., Fan P., Yan C., Willers H, & Zhang J. (2015). 53BP1 promotes microhomology-mediated end-joining in G1-phase cells. Nucleic Acids Research, 43(3), 1659-1670.

Publication 2015

Rabbit anti-53BP1 Mouse anti-BRCA1 (D-9, HA.11 clone 16B12 Mre11

53bp1 Anti igg Antibodies Biologicals Brca1 Cell extracts Clone Dilutions Goat Horseradish peroxidase Mouse Novus Proteins Rabbit Sodium dodecyl sulphate polyacrylamide gel electrophoresis

Corresponding Organization : Case Western Reserve University

Other organizations : Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Georgia Regents Medical Center, Augusta University, Massachusetts General Hospital

Top 5 similar protocols

Variable analysis

independent variables

Rabbit anti-53BP1 antibody
Mouse anti-BRCA1 (D-9, Santa Cruz) antibody
HA.11 clone 16B12 from Covance antibody
Mre11 antibody
Goat-anti-mouse IgG–horseradish peroxidase (HRP) conjugated antibody
Goat-anti-rabbit IgG–HRP conjugated antibody

dependent variables

Protein expression levels of 53BP1, BRCA1, and Mre11

control variables

Cell extracts were prepared and proteins were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis as described previously (47, 54)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!