Quantifying Pulmonary Barrier Permeability

Protein and albumin content, a measure to quantitate increased permeability of the bronchoalveolar–capillarity barrier, and lactate dehydrogenase (LDH) activity, an indicator of general cytotoxicity, were determined in the acellular BAL fluid [15 (link)]. Protein content was measured by the bicinchoninic (BCA) protein assay (Pierce Biotechnology Inc., Rockford, IL). Albumin content was determined colorimetrically based on albumin binding to bromcresol green using an albumin diagnostic kit (Wiener Lab, Buenos Aires, Argentina). LDH activity, expressed as units per liter of BAL fluid, was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide (NAD) using the Wiener reagents and procedures (Wiener Lab).

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Villena J., Chiba E., Tomosada Y., Salva S., Marranzino G., Kitazawa H, & Alvarez S. (2012). Orally administered Lactobacillus rhamnosus modulates the respiratory immune response triggered by the viral pathogen-associated molecular pattern poly(I:C). BMC Immunology, 13, 53.

Publication 2012

Albumin Assay Bromcresol green Capillarity permeability Cytotoxicity Diagnostic Lactate dehydrogenase Protein Reduced nicotinamide adenine dinucleotide

Corresponding Organization :

Other organizations : Tohoku University, Consejo Nacional de Investigaciones Científicas y Técnicas

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Protocol cited in 7 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein content
Albumin content
Lactate dehydrogenase (LDH) activity

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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