We performed antimicrobial susceptibility testing (AST) on 5 Campylobacter coli and 39 Campylobacter jejuni isolates recovered from the litter of broiler chicks following the National Antimicrobial Resistance Monitoring System (NARMS) protocol for Gram-negative bacteria. The following antimicrobials were tested: Azithromycin, Ciprofloxacin, Clindamycin, Erythromycin, Florfenicol, Gentamicin, Nalidixic Acid, Telithromycin and Tetracycline. Antimicrobial susceptibility of Campylobacter isolates was determined using the Sensititre semi-automated system (Thermo Fisher Scientific, Kansas City, KS) according to manufacturer’s instructions. Briefly, bacterial suspensions equivalent to a 0.5 McFarland suspension were prepared, aliquoted into a CAMPY panel and incubated at 42°C for 24 h under microaerobic conditions. Minimum inhibitory concentrations were determined and categorized as resistant according to breakpoints based on epidemiological cut-off values as used by the National Antimicrobial Resistance Monitoring System (NARMS; https://www.fda.gov/media/108180/download ).
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Antimicrobial Susceptibility of Campylobacter
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Antimicrobial
Azithromycin
Bacterial
Campylobacter
Campylobacter coli
Campylobacter jejuni
Ciprofloxacin
Clindamycin
Erythromycin
Florfenicol
Gentamicin
Gram negative bacteria
H conditions
Minimum inhibitory concentrations
Nalidixic acid
Susceptibility
Telithromycin
Tetracycline
Corresponding Organization : U.S. National Poultry Research Center
Other organizations : Southeast Watershed Research Laboratory
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Variable analysis
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- Antimicrobial agents tested: Azithromycin, Ciprofloxacin, Clindamycin, Erythromycin, Florfenicol, Gentamicin, Nalidixic Acid, Telithromycin and Tetracycline
- Antimicrobial susceptibility of Campylobacter isolates
- Bacterial suspensions prepared to a 0.5 McFarland standard
- Incubation at 42°C for 24 h under microaerobic conditions
- Minimum inhibitory concentrations (MICs) determined and categorized as resistant according to NARMS breakpoints
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