Prostate Cancer Protein Regulation

anti-AR (Santa Cruz Biotechnology; N20 and C19 clones), anti-AR-v7 (Precision Antibodies), anti-H2A.Z (19 (link)), anti-MDM2 (Santa Cruz N20 and SMP14), anti-HA (Santa Cruz Biotechnology; Y11 clone), anti-FLAG (Sigma), anti-p53 (Dako), anti-α-tubulin (Sigma), and anti-ubiquitin (Santa Cruz Biotechnology) were included in this project. Plasmids used were pPSA-Luc, pARE3-Luc, pCMV-β-gal, pHA-ubiquitin, pMYC-MDM2, pFLAG-His-AR (20 (link)) and pFLAG-His-AR-K311R and pFLAG-V7-AR mutants generated by in vitro mutagenesis (QuikChange; Stratagene). Lentiviral AR careers were prepared as previously described (21 (link)).

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McClurg U.L., Cork D.M., Darby S., Ryan-Munden C.A., Nakjang S., Mendes Côrtes L., Treumann A., Gaughan L, & Robson C.N. (2016). Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity. Nucleic Acids Research, 45(4), 1793-1804.

Publication 2016

anti-AR anti-MDM2 anti-HA anti-FLAG anti-p53 anti-α-tubulin anti-ubiquitin

Antibodies Clone Mdm2 Mutagenesis Plasmids Ubiquitin α tubulin

Corresponding Organization : Newcastle University

Top 5 similar protocols

Variable analysis

independent variables

Anti-AR (Santa Cruz Biotechnology; N20 and C19 clones)
Anti-AR-v7 (Precision Antibodies)
Anti-H2A.Z (19 (link))
Anti-MDM2 (Santa Cruz N20 and SMP14)
Anti-HA (Santa Cruz Biotechnology; Y11 clone)
Anti-FLAG (Sigma)
Anti-p53 (Dako)
Anti-α-tubulin (Sigma)
Anti-ubiquitin (Santa Cruz Biotechnology)
PPSA-Luc
PARE3-Luc
PCMV-β-gal
PHA-ubiquitin
PMYC-MDM2
PFLAG-His-AR (20 (link))
PFLAG-His-AR-K311R
PFLAG-V7-AR mutants generated by in vitro mutagenesis (QuikChange; Stratagene)
Lentiviral AR careers

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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