Human trophoblast cell lines (Jeg3, HTR8) and human decidual stromal cell line (hESC) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Jeg3 and HTR8 cells were cultured in a 5% humidified carbon dioxide atmosphere at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Gibco, Life Technologies, Grand Island, NY, USA) with 10% foetal bovine serum (Gibco, Life Technologies, Grand Island, NY, USA), 50 mg/mL streptomycin, and 50 U/mL penicillin. Jeg3 is the only trophoblast cell line that expresses human leukocyte antigen G (HLA-G), which is gradually expressed during development from CTBs to EVTs.
hESCs were cultured in a 5% humidified carbon dioxide atmosphere at 37 °C in phenol red-free Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Meilunbio, Dalian, China) with 10% foetal bovine serum, 50 mg/mL streptomycin, and 50 U/mL penicillin. One micromolar medroxyprogesterone-17-acetate (MPA) (HY-B0469, MedChemExpress, NJ, USA) and 0.5 mM N6,20-O-dibutyryladenosine cAMP sodium salt (db-cAMP) (HY-B0764, MedChemExpress, Monmouth Junction, NJ, USA) were added to the culture for 6 days to induce hESC decidualization in vitro [14 (link)].
hESCs were cultured in a 5% humidified carbon dioxide atmosphere at 37 °C in phenol red-free Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Meilunbio, Dalian, China) with 10% foetal bovine serum, 50 mg/mL streptomycin, and 50 U/mL penicillin. One micromolar medroxyprogesterone-17-acetate (MPA) (HY-B0469, MedChemExpress, NJ, USA) and 0.5 mM N6,20-O-dibutyryladenosine cAMP sodium salt (db-cAMP) (HY-B0764, MedChemExpress, Monmouth Junction, NJ, USA) were added to the culture for 6 days to induce hESC decidualization in vitro [14 (link)].
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