DNA Methylation Induction in Colorectal Cancer Cells

The LoVo and Caco-2 were obtained from American Type Culture Collection (ATCC), USA. Cells were grown in DMEM (Sigma) media containing 10% FBS and 10000 U/ml antibiotic and then kept at 37°C in 5% CO₂ incubator. LoVo and Caco-2 were treated with 1 μM 5-aza-2′-deoxycytidine (Sigma) for 72 hours, and a medium was replaced every 24 hours. Control cultures had an equal volumes of drug solvent (DMSO) [12 (link)].

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Almutairi B.O., Almutairi M.H., Alrefaei A.F., Ali D., Alkahtani S, & Alarifi S. (2022). Cigarette Smoke Regulates the Expression of EYA4 via Alternation of DNA Methylation Status. BioMed Research International, 2022, 5032172.

Publication 2022

Caco-2 DMEM 5-aza-2′-deoxycytidine

Antibiotic Cells Deoxycytidine Drug Solvent

Corresponding Organization : King Saud University

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Variable analysis

independent variables

Treatment with 1 μM 5-aza-2′-deoxycytidine (Sigma) for 72 hours

dependent variables

Not explicitly mentioned

control variables

DMEM (Sigma) media containing 10% FBS and 10000 U/ml antibiotic
Kept at 37°C in 5% CO2 incubator

positive control

Control cultures had an equal volumes of drug solvent (DMSO)

negative control

Not explicitly mentioned

Annotations

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