Isolation of total RNA using the Direct-zol™ RNA kit (Zymo Research, Orange, CA, USA) and synthesis of cDNA using the iScript™ cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA) followed the manufacturer’s protocol. The transcripts were amplified using the SensiFAST SYBR qPCR mix (Bioline, London, UK) [31 (link)]. Table 1 shows the primers used for amplification.
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