Microstructural Analysis of Murine Bone Specimens

Femurs and tibias were scanned after fixation in paraformaldehyde. The μCT scans were performed using a nanotom® m (phoenix|x-ray, GE Sensing & Inspection Technologies GmbH, Wunstorf, Germany) equipped with a 180 kV/15 W nanofocus X-ray source. A tungsten transmission target, an accelerating voltage of 70 kV, and a beam current of 260 μA were used. To increase mean energy of the photon spectrum and consequently reduce beam hardening artefacts, a 0.5 mm aluminium filter was inserted between source and specimen. A region of air was designated in all scans as suggested by the software (GE Sensing & Inspection Technologies GmbH) to standardize the grey-scale for data interpretation. 1440 equiangular projection images were acquired over 360° with an exposure time of 1 second. The radiographs were reconstructed using a cone beam filtered back-projection algorithm with the manufacturer's software phoenix datos|x 2.0.1 RTM (GE Sensing & Inspection Technologies GmbH). Whole bones were scanned and reconstructed with a voxel size of 18.5 μm. Datasets were visualised using VG Studio MAX 2.1 (Volume Graphics GmbH, Heidelberg, Germany) and analysed using ImageJ with the BoneJ and 3D Shape plugins [20 (link)–22 (link)].

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Sutter S., Todorov A., Ismail T., Haumer A., Fulco I., Schulz G., Scherberich A., Kaempfen A., Martin I, & Schaefer D.J. (2017). Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging, 2017, 4035160.

Publication 2017

VG Studio MAX 2.1

Aluminium Beam Bones Cone Femurs Paraformaldehyde Radiographs Tibias Transmission Tungsten X ray

Corresponding Organization : University Hospital of Basel

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Variable analysis

independent variables

Fixation of femurs and tibias in paraformaldehyde

dependent variables

Morphological properties of femurs and tibias measured using μCT scans

control variables

Use of a nanotom® m μCT scanner with a 180 kV/15 W nanofocus X-ray source
Accelerating voltage of 70 kV and beam current of 260 μA
Use of a 0.5 mm aluminium filter to reduce beam hardening artifacts
Designation of a region of air in all scans to standardize the grey-scale
Acquisition of 1440 equiangular projection images over 360° with an exposure time of 1 second
Reconstruction of whole bones with a voxel size of 18.5 μm

positive controls

None specified

negative controls

None specified

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