Immunofluorescence Staining for Neuronal Markers

The following primary antibodies were used for immunofluorescence staining: goat anti-tdTomato (1:500; catalog #AB8181-200, SICGEN; RRID:AB_2722750); rabbit anti-TRP73 (1:500; catalog #ab40658, Abcam; RRID:AB_776999); mouse anti-RELN (1:500; catalog #MAB5364, MilliporeSigma; RRID:AB_1293544); mouse anti-RELN (1:500; catalog #ab78540, Abcam; RRID:AB_1603148); rabbit anti-CALB1 (1:500; catalog #CB38, Swant; RRID:AB_10000340); mouse anti-DCX (1:25; catalog #sc-271390, Santa Cruz Biotechnology; RRID:AB_10610966); and goat anti-EGFP/YFP (yellow fluorescent protein; 1:500; catalog #AB0020-500, SICGEN; RRID:AB_2333100). The secondary antibodies used were as follows: donkey anti-goat Alexa Fluor 555 (1:1000; catalog #A21432, Thermo Fisher Scientific; RRID:AB_2535853); donkey anti-rabbit Alexa Fluor 488 (1:1000; catalog #A21206, Thermo Fisher Scientific; RRID:AB_2535792); and donkey anti-mouse Alexa Fluor 647 (1:1000; catalog #A31571, Thermo Fisher Scientific; RRID:AB_162542).

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van Bruggen R., Patel Z.H., Wang M., Suk T.R., Rousseaux M.W, & Tan Q. (2023). A Versatile Strategy for Genetic Manipulation of Cajal–Retzius Cells in the Adult Mouse Hippocampus. eNeuro, 10(10), ENEURO.0054-23.2023.

Publication 2023

ab40658 ab78540 sc-271390 A21432 A21206 A31571

Alexa fluor 488 Alexa fluor 555 Alexa fluor 647 Antibodies Donkey Goat Immunofluorescence Mouse Protein Rabbit Reln Tdtomato

Corresponding Organization :

Other organizations : University of Alberta, University of Ottawa

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