Culturing Human Umbilical Vein Endothelial Cells

Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Allendale, NJ) and cultured in endothelial cell growth medium-2 (EGM-2; Lonza) bullet kit. Vero cells and lenti-X-293T cells were obtained from Korean Cell Line Bank (Seoul, South Korea) and Takara (Otsu, Japan), respectively. They were cultured in Dulbecco's modified Eagle's medium (DMEM; GE Healthcare, Little Chalfont, UK) supplemented with 10% fetal bovine serum (FBS; Wellgene, Seoul, South Korea) and 1% antibiotics (Lonza). The cells were maintained in a humidified atmosphere of 5% CO₂ at 37°C according to the manufacturer's instructions. An absence of contamination of mycoplasma in all cultured cells was tested by mycoplasma detecting PCR every month as described previously (11 (link)).