LysECD7 protein (NCBI AN: YP_009602067.1) was obtained as described before [20 (link)], synthetic gene was used for investigations. Briefly, LysECD7′s initial coding sequence was artificially synthesized in a pAL-TA commercial vector (Evrogen Ltd., Moscow, Russia). Thereafter endolysin ORF was amplified from a pALTA-LysECD7 clone and integrated into the expression vector pET-42b(+) (Evrogen Ltd., Moscow, Russia), resulting in a pET42b-LysECD7-8his plasmid. All constructs were checked for errors via Sanger sequencing.
Clone Coding sequenceEndolysin Plasmid Protein Synthetic gene Vector
Corresponding Organization : Gabrichevsky Institute of Epidemiology and Microbiology
Other organizations :
State Research Center for Applied Microbiology and Biotechnology, Astrakhan State Medical University, A N Bach Institute of Biochemistry, Center for Strategic Research, Sechenov University
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