Evaluation of Pro-Inflammatory Protein Levels

The expression levels of the pro-inflammatory proteins iNOS and COX-2 were performed as described above [53 (link)]. Cell lysis was lysed with Tris-HCl buffer (20 mM, pH 7.4) containing the protease inhibitor mixture (5 mg/mL pepstatin A, 5 mg/mL aprotinin, 1 mg/mL chymostatin and 0.1 mM phenylmethanesulfonyl fluoride). The kit used for protein concentration determination was the Lowry Protein Assay Kit (Sigma-Aldrich, Saint Louis, MO, USA). Antibodies used were iNOS (Cayman Chemical, Ann Arbor, MI, USA) and Anti-COX2/Cyclooxygenase 2 (Abcam, Cambridge, UK).

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Yoon C.S., Lee H., Liu Z., Lee H.K, & Lee D.S. (2022). Effects of Compounds Isolated from Lindera erythrocarpa on Anti-Inflammatory and Anti-Neuroinflammatory Action in BV2 Microglia and RAW264.7 Macrophage. International Journal of Molecular Sciences, 23(13), 7122.

Publication 2022

Lowry Protein Assay Kit Anti-COX2/Cyclooxygenase 2

Antibodies Aprotinin Assay Cayman Cell Chymostatin Cox 2 Cox2 cyclooxygenase Inflammatory Inos Pepstatin Phenylmethanesulfonyl fluoride Pro proteins Protease inhibitor Protein Tris buffer

Corresponding Organization : Chosun University

Other organizations : Korea Research Institute of Bioscience and Biotechnology, University of Florida

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression levels of the pro-inflammatory proteins iNOS and COX-2

control variables

Cell lysis with Tris-HCl buffer (20 mM, pH 7.4) containing the protease inhibitor mixture (5 mg/mL pepstatin A, 5 mg/mL aprotinin, 1 mg/mL chymostatin and 0.1 mM phenylmethanesulfonyl fluoride)
Use of the Lowry Protein Assay Kit (Sigma-Aldrich, Saint Louis, MO, USA) for protein concentration determination
Use of iNOS (Cayman Chemical, Ann Arbor, MI, USA) and Anti-COX2/Cyclooxygenase 2 (Abcam, Cambridge, UK) antibodies

controls

No positive or negative controls are explicitly mentioned in the information provided.

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