ApoE–/– mice were purchased from Qingzilan Technology Co., Ltd. (Nanjing, China), and New Zealand rabbits were acquired from Fuwai Hospital (Beijing, China). C-18 light Sep-Pak cartridges and QMA light ion-exchange cartridges were obtained from Waters (Milford, MA, United States). The 18F isotope was acquired through Jiangsu Huayi Technology Co., Ltd. (Changshu, China).
The scaffolds and 18F-labeled ASEM were synthesized and followed the descriptions in several previous studies (Gao et al., 2013 (link); Wang et al., 2021 (link)). Briefly, the ortho-substitution amino was countered with nucleophilic radio-fluorination in the last Sandmeyer reaction. Then, the product was purified by high-performance liquid chromatography (HPLC) and diluted through a C18 column. The radiochemical purity was > 99%.
The scaffolds and 18F-labeled ASEM were synthesized and followed the descriptions in several previous studies (Gao et al., 2013 (link); Wang et al., 2021 (link)). Briefly, the ortho-substitution amino was countered with nucleophilic radio-fluorination in the last Sandmeyer reaction. Then, the product was purified by high-performance liquid chromatography (HPLC) and diluted through a C18 column. The radiochemical purity was > 99%.
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