Quantifying Fascin Expression in Cancer

Example 3

The levels of fascin mRNA and protein can be determined by real-time PCR and Western blot, respectively. For quantitative real-time PCR, samples from cancer patients were used for RNA isolation. Oligonucleotide primers specific for fascin mRNA were used for PCR reactions. For Western blots, samples from cancer patients were assessed with anti-fascin antibody. The intensity of the bands representing fascin proteins was quantified by image documentation and quantification software.

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US11866440B2. Methods for inhibiting fascin (2024-01-09). NOVITA PHARMACEUTICALS, INC. [US], CORNELL UNIVERSITY [US]. Inventors: Xin-Yun Huang [US], Christy Young Shue [US].

Patent 2024

Anti antibody Cancer Fascin Isolation Mrna Oligonucleotide primers Patients Proteins Quantitative real time pcr Western blot Western blots

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of fascin mRNA
Levels of fascin protein

control variables

None explicitly mentioned

controls

Positive control: Samples from cancer patients used for RNA isolation and Western blot analysis
Negative control: Not explicitly mentioned

Annotations

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