Western Blot Analysis of Protein Markers

WB analysis was carried out as previously described^{14 ,34 (link)}. The following antibodies were used in these assays: NGFR (C-20, Santa Cruz Biotechnology and D4B3, Cell Signaling Technology, Danvers, MA, USA), SOX2 (D6D9, Cell signaling Technology, Danvers, MA, USA), p53 (DO-1, Santa Cruz Biotechnology), p21 (CP74, Neomarkers, Fremont, CA, USA), PUMA (H-136, Santa Cruz, Biotechnology), and MDM2 (SMP14, Santa Cruz Biotechnology, 2A9, 2A10, and 4B11).

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Jiang L., Huang S., Wang J., Zhang Y., Xiong Y., Zeng S.X, & Lu H. (2020). Inactivating p53 is essential for nerve growth factor receptor to promote melanoma-initiating cell-stemmed tumorigenesis. Cell Death & Disease, 11(7), 550.

Publication 2020

SOX2 (D6D9, p53 (DO-1,

Antibodies Assays Mdm2 Ngfr Puma Sox2

Corresponding Organization :

Other organizations : Tulane University, Nanchang University

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Variable analysis

independent variables

Antibodies used in Western blot (WB) analysis: NGFR (C-20, Santa Cruz Biotechnology and D4B3, Cell Signaling Technology, Danvers, MA, USA), SOX2 (D6D9, Cell signaling Technology, Danvers, MA, USA), p53 (DO-1, Santa Cruz Biotechnology), p21 (CP74, Neomarkers, Fremont, CA, USA), PUMA (H-136, Santa Cruz, Biotechnology), and MDM2 (SMP14, Santa Cruz Biotechnology, 2A9, 2A10, and 4B11)

dependent variables

Protein expression levels measured by Western blot analysis

control variables

Not explicitly mentioned

controls

Positive and negative controls for Western blot analysis, as described in the referenced studies, were not specified in the provided information.

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