Three-dimensional reconstruction of biological samples

Living specimens were photographed with a Canon 600D Camera mounted on a Zeiss- Stemi 2000. Parafin- and semithin sections were analyzed with an Olympus microscope (BX-51). Sections were photographed with an Olympus camera (Olympus cc12) using the dot slide system (2.2 Olympus, Hamburg) and aligned using imod [58 (link)] and imod align (http://www.q-terra.de/biowelt/3drekon/guides/imod_first_aid.pdf). 3D reconstructions were performed with Fiji (1.45b) [57 (link)]/trakem [59 (link)] and Amira (5.0). Adobe (San Jose, CA, USA) Photoshop (CC) and Illustrator (CS6/CC) were used to prepare all figures.

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Beckers P., Helm C, & Bartolomaeus T. (2019). The anatomy and development of the nervous system in Magelonidae (Annelida) – insights into the evolution of the annelid brain. BMC Evolutionary Biology, 19, 173.

Publication 2019

600D Camera dot slide system Illustrator (CS6/CC)

First aid Imod Microscope Reconstructions Stemi

Corresponding Organization : University of Göttingen

Other organizations : University of Bonn

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Variable analysis

independent variables

Imaging equipment used (Canon 600D Camera, Zeiss-Stemi 2000, Olympus microscope BX-51, Olympus camera Olympus cc12, dot slide system 2.2, imod/imod align software, Fiji/trakem, Amira 5.0, Adobe Photoshop/Illustrator)

dependent variables

Characteristics of living specimens and tissue sections observed and analyzed through the various imaging techniques

control variables

Not explicitly mentioned

controls

No positive or negative controls specified

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