Serum Lipid and DNA Extraction Protocol

Fasting venous blood samples of 5 ml were collected from each subject. A portion of the sample (2 ml) was placed in a tube and used to measure serum lipid levels. The remaining sample of 3 ml was collected in a glass tube containing anticoagulants (14.70 g/L glucose, 13.20 g/L trisodium citrate, 4.80 g/L citric acid) and utilized to extract deoxyribonucleic acid (DNA). The methods for performing serum ApoA1, HDL-C, ApoB, TG, LDL-C and TC measurements were described in a previous study^{29 (link)}. All determinations were conducted using an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University^{30 (link),31 (link)}.

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Zheng P.F., Yin R.X., Wei B.L., Liu C.X., Deng G.X, & Guan Y.Z. (2020). Associations of PRKN–PACRG SNPs and G × G and G × E interactions with the risk of hyperlipidaemia. Scientific Reports, 10, 13010.

Publication 2020

Type 7170A

Anticoagulants Apoa1 Apob Blood Citric acid Deoxyribonucleic acid Glucose Lipid Serum Trisodium citrate Venous

Corresponding Organization :

Other organizations : First Affiliated Hospital of GuangXi Medical University, Guangxi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum lipid levels (ApoA1, HDL-C, ApoB, TG, LDL-C, TC)

control variables

Fasting venous blood samples of 5 ml were collected from each subject
A portion of the sample (2 ml) was placed in a tube and used to measure serum lipid levels
The remaining sample of 3 ml was collected in a glass tube containing anticoagulants (14.70 g/L glucose, 13.20 g/L trisodium citrate, 4.80 g/L citric acid) and utilized to extract deoxyribonucleic acid (DNA)
All determinations were conducted using an autoanalyzer (Type 7170A; Hitachi Ltd., Tokyo, Japan) in the Clinical Science Experiment Center of the First Affiliated Hospital, Guangxi Medical University

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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