Schwann Cell Migration Assay

SC migration was assessed using a transwell-based assay as previously described (Parrinello et al., 2010 (link)). In brief, SCs were seeded onto the upper chamber of a transwell insert with 8 μm pores (Costar, Cambridge, MA) at a cell density of 1 × 10⁶ cells/mL in DMEM. To evaluate SC migration, the cells were allowed to migrate at 37°C in 5% CO₂ for 24 h, after which the transwell insert was removed and the upper surface of its insert membrane cleaned with a cotton swab. The cells on the bottom surface of the insert membrane were next stained with 0.1% crystal violet for 30 min at room temperature and subsequently imaged and counted under a DMR inverted microscope (Leica).