Western Blot Analysis of Cellular Markers

Western blotting was conducted as previously described 34 (link). The following primary antibodies were used in this study: GRP78 (# 3177; Cell Signaling Technology), GRP94 (#3196-1; Epitomics), pERK1/2 (#sc-377400, Santa Cruz Biotechnology), p65 (#sc-8008; Santa Cruz Biotechnology), CHOP(#2895; Cell Signaling Technology), cleaved PARP (#sc-56196, Santa Cruz Biotechnology), NLRP3 (#sc-134306; Santa Cruz Biotechnology), HXK2 (#sc-374091, Santa Cruz Biotechnology), IL-1β (#sc-12742; Santa Cruz Biotechnology), IL-18 (#sc133127; Santa Cruz Biotechnology), p-eIF2a (#33981; Cell Signaling Technology), Casp1 (#YH050707C; Epitonics), ZDHHC1 (#AP10315a; Abgent), E-cadherin (#sc8426; Santa Cruz Biotechnology), Occludin (#TA306787; Origene), N-cadherin (#610920; BD transduction laboratories), Vimentin (#5741s; Cell Signaling Technology), G6PD (#12263s; Cell Signaling Technology), Casp3 (#9664; Cell Signaling Technology), Casp7 (#8438; Cell Signaling Technology), GLUT1(#sc-377228; Santa Cruz Biotechnology), beta-actin (#sc8432; Santa Cruz Biotechnology), GAPDH (#sc-47727; Santa Cruz Biotechnology).

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Le X., Mu J., Peng W., Tang J., Xiang Q., Tian S., Feng Y., He S., Qiu Z., Ren G., Huang A., Lin Y., Tao Q, & Xiang T. (2020). DNA methylation downregulated ZDHHC1 suppresses tumor growth by altering cellular metabolism and inducing oxidative/ER stress-mediated apoptosis and pyroptosis. Theranostics, 10(21), 9495-9511.

Publication 2020

GRP78 pERK1/2 cleaved PARP NLRP3 IL-1β IL-18 p-eIF2a E-cadherin Occludin N-cadherin Vimentin Casp3 Casp7 GLUT1 beta-actin GAPDH

Antibodies Beta actin Casp3 Casp7 Chop E cadherin G6pd Gapdh Glut1 Grp78 Grp94 Il 18 Il 1β N cadherin Occludin Vimentin

Corresponding Organization :

Other organizations : Chongqing Medical University, First Affiliated Hospital of Chongqing Medical University, Lovelace Respiratory Research Institute, Chinese University of Hong Kong

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

The expression levels of the following proteins: GRP78, GRP94, pERK1/2, p65, CHOP, cleaved PARP, NLRP3, HXK2, IL-1β, IL-18, p-eIF2a, Casp1, ZDHHC1, E-cadherin, Occludin, N-cadherin, Vimentin, G6PD, Casp3, Casp7, GLUT1, beta-actin, GAPDH

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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