THP-1 cells were obtained from the American Type Culture Collection and expanded in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, Darmstadt, Germany), 1% penicillin/streptomycin (Lonza, Basel, Switzerland), 1% amphotericin B (Biochrom AG, Berlin, Germany) and 0.05 nM 2-mercaptoethanol (Sigma-Aldrich, Darmstadt, Germany).
After approval by the local ethics committee (No. 618/2017BO2), JPCs from three donors were included in this study. JPCs were cultured and expanded with DMEM/F12 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 5% hPL (provided by the Institute for Clinical and Experimental Transfusion Medicine of the University Hospital Tübingen), 1% penicillin/streptomycin (Lonza, Basel, Switzerland) and 1% amphotericin B (Biochrom AG, Berlin, Germany). Cell passaging of JPCs was performed with TrypLE-Express (Thermo Fisher Scientific, Waltham, MA, USA). Passage 4 of JPCs was used to culture on β-TCP scaffolds (Curasan AG, Kleinostheim, Germany). Details concerning the fabrication process are provided in the previous publication [42 (link)].
After approval by the local ethics committee (No. 618/2017BO2), JPCs from three donors were included in this study. JPCs were cultured and expanded with DMEM/F12 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 5% hPL (provided by the Institute for Clinical and Experimental Transfusion Medicine of the University Hospital Tübingen), 1% penicillin/streptomycin (Lonza, Basel, Switzerland) and 1% amphotericin B (Biochrom AG, Berlin, Germany). Cell passaging of JPCs was performed with TrypLE-Express (Thermo Fisher Scientific, Waltham, MA, USA). Passage 4 of JPCs was used to culture on β-TCP scaffolds (Curasan AG, Kleinostheim, Germany). Details concerning the fabrication process are provided in the previous publication [42 (link)].
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