Antimicrobial-resistant isolates were identified using bacterial 16S rRNA gene sequence analysis. For amplification of the 16S rRNA genes, isolates were cultured in Tryptic soy broth (TSB2, BBL BD Becton Dickinson™) with 2% NaCl at 22 °C for 12–24 h and centrifuged at 9000× g for 3 min using an Eppendorf 5415D (Eppendorf AG, Hamburg, Germany) microcentrifuge to obtain a pellet. DNA extraction was carried out using the Wizard® Genomic DNA Purification commercial kit (Promega, Madison, WI, USA) following the supplier’s instructions, and the obtained DNA samples were stored at −20 °C until analysis.
The amplification of the 16S ribosomal genes of the isolates was carried out using PCR, following the methodology described by Opazo et al. [37 (link)]. The resulting amplified PCR products were sequenced with Macrogen (Rockville, MD, USA), using the ABI PRISM 373 DNA Sequencer (Applied Biosystems, Foster City, CA, USA). The sequences were edited and matched to the Ribosomal Database Project (http://rdp.cme.msu.edu/ , accessed on 5 January 2024) to identify the bacterial isolates. The partial sequences of the 16S rDNA gene belonging to each isolate were deposited in the GenBank database under the accession numbers shown in Tables S1 and S2 (Supplementary Materials) .
The amplification of the 16S ribosomal genes of the isolates was carried out using PCR, following the methodology described by Opazo et al. [37 (link)]. The resulting amplified PCR products were sequenced with Macrogen (Rockville, MD, USA), using the ABI PRISM 373 DNA Sequencer (Applied Biosystems, Foster City, CA, USA). The sequences were edited and matched to the Ribosomal Database Project (
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