Patch Clamp Recording of Dorsal Root Ganglion Neurons

Patch pipettes (World Precision Instruments) were pulled to a fine diameter with a resistance of 3–5 MΩ. The pipettes were backfilled with intracellular solution containing (in mM) 150 KCl, 3 MgCl₂·6H2O, 5 ethylene glycol tetraacetic acid (EGTA), 10 HEPES (adjusted to pH 7.25 with KOH). The cells were recorded as previously described with constant perfusion (2 mL/min) of extracellular solution containing (in mM) 150 NaCl, 5 KCl, 3 MgCl₂·6H2O, 1 CaCl₂, 10 HEPES, 10 glucose (adjusted to pH 7.4 with NaOH).^{48 (link)} Where indicated small molecules at the concentrations listed were added to the extracellular solution. For DRG recordings, neurons were incubated with Alexa Fluor 488 conjugated isolectin GS-IB4 (Invitrogen) as previously reported, and green cells were recorded.^{3 (link)}