Wild-type laboratory isolate WMD and multidrug-resistant isolate KGR were described previously (Kitchen et al., 2019 (link)). The KGR isolate used here is resistant to Thiabendazole (TBZ) and IVM. Multidrug-resistant BCR was isolated from a retired racing greyhound, which was raised in Kansas and raced in Florida, in October 2019. The dog had a history of recurring hookworm infections, and had been treated with Drontal Plus (praziquantel/pyrantel pamoate/febantel) prior to adoption. Infective third-stage larvae (iL3s) reared from eggs collected from feces of the infected dog were used to infect a hookworm naïve beagle. All isolates were maintained in beagles as described previously (Schad, 1982 ; Krepp et al., 2011 (link)). Thiabendazole, ivermectin, pyrantel pamoate, dimethyl sulfoxide (DMSO) and 8-bromo-cGMP were purchased from Sigma-Aldrich (St. Louis, MO, USA). Moxidectin (MOX) was a gift from Zoetis (Kalamazoo, MI, USA).
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