Protocol detail

Multi-Epitope Protein Purification

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The epitopes were grouped according to the protein of origin, in the following order: Envelope, NS1, and NS3. The selected epitopes were joined together with the linker GPGPG. At the amino-terminal four additional residues (MGGS) were added for cloning purposes. Finally, an RSHHHHHH polyhistidine tail was added at the carboxyterminal of the protein to enable purification by Immobilized Metal ion Affinity Chromatography (IMAC).²³

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da Silva I.B., de Moraes Rodrigues J., Batista R.C., Gomes V.D., Chacon C.D., Almeida M.D., de Araujo T.S., Ortiz da Silva B., Castiñeiras T.M., Ferreira Junior O.D., Carneiro F.A, & Montero-Lomeli M. (2024). Development and assessment of a multiepitope synthetic antigen for the diagnosis of Dengue virus infection. The Brazilian Journal of Infectious Diseases, 28(3), 103746.

Publication 2024

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Variable analysis

independent variables

Protein of origin (Envelope, NS1, and NS3)

dependent variables

Epitopes selected and joined together with the linker GPGPG
Addition of MGGS at the amino-terminal for cloning purposes
Addition of RSHHHHHH polyhistidine tail at the carboxyterminal for purification by IMAC

control variables

Linker GPGPG used to join the selected epitopes
Polyhistidine tail RSHHHHHH added for purification by IMAC

Annotations

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