Visualizing Cas13 Protein Expression in N. benthamiana

For the expression analysis of different Cas13 proteins, the epidermis of agroinfiltrated N. benthamiana leaves was peeled off. GFP fluorescence was visualized under an Olympus BX63 epifluorescence microscope. For the observation of transiently expressed mGFP5, TuMV‐expressed GFP, and CMV‐expressed DsRed in the ILs, the agroinfiltrated leaves were cut off after 4, 6, and 9 days postinfiltration (dpi) in different experiments. The corresponding fluorescence was excited by different handheld fluorescent excitation lamps (blue or green light) and photographed with different filters in the dark (Aman et al., 2018 (link); Mahas et al., 2019 (link)). For the observation of TuMV‐expressed GFP or CMV‐expressed DsRed in the SLs, the whole plants were photographed at 6 or 9 dpi (Aman et al., 2018 (link); Mahas et al., 2019 (link)).