The RNA immunoprecipitation (RIP) assay was performed using a RIP Kit (Millipore, Burlington, MA, USA). In brief, 5 µg anti-hnRNPK or anti-FLAG antibodies and magnetic beads were mixed and incubated with cells lysed with RIP lysis buffer supplemented with protease and RNase inhibitors overnight at 4 °C. The immunoprecipitated RNA was obtained for qRT-PCR after digestion with proteinase K buffer.
Coimmunoprecipitation assay was performed with an IP/Co-IP Kit (#88,828; Thermo Fisher Scientific) to determine the interactions of hnRNPK with p53 and SENP2. The detailed procedures were performed as previously described [22 (link)].
Coimmunoprecipitation assay was performed with an IP/Co-IP Kit (#88,828; Thermo Fisher Scientific) to determine the interactions of hnRNPK with p53 and SENP2. The detailed procedures were performed as previously described [22 (link)].
Free full text: Click here
