Evaluating Parasitic Infection Reduction

Using the SPSS software package for Windows (version 22) [23 ], descriptive statistics for body condition score, FAMACHA score, and faecal egg count was calculated. To compare values of investigated variables between day 0 and day 12 for each group, a Wilcoxon signed-rank test for dependent samples was used. Faecal egg counts among the groups before and after supplementation were compared using an independent samples Kruskal-Wallis test and Dunett’s T3 post hoc test.
To determine the percentage of Strongylid eggs shedding reduction in samples after the supplementation, we calculated the mean number of eggs in the samples from each group before supplementation (T1) and after supplementation (T2), and used the equation [25 (link), 27 ]:
$$\mathrm{Egg} \mathrm{reduction} \left(\%\right)=100\times \left(1-⌈\frac{\mathrm{T}2}{\mathrm{T}1}⌉\right).$$
The 95% confidence intervals were calculated with the eggCounts on-line analysis program [25 (link), 46 (link)], using the Two samples paired model procedure (http://shiny.math.uzh.ch/user/furrer/shinyas/shiny-eggCounts/).
Eggs of other parasites and coccidian oocysts were found only in small numbers in some animals and were therefore not included in the statistical analysis.