Zebrafish Larval EEG for Epileptiform Discharges

The EEG recordings were performed as previously described by Afrikanova et al. [45 (link)]. Epileptiform-like discharges were detected by inserting a glass electrode filled with artificial cerebrospinal fluid (124 mM NaCl, 2 mM KCl, 2 mM MgSO₄, 2 Mm CaCl₂, 1.25 mM KH₂PO₄, 26 mM NaHCO₃, 10 mM glucose) into the optic tectum of individual 6-dpf zebrafish larvae for 20 min (MultiClamp 700B amplifier, Digidata 1550 digitiser, Axon instruments, USA). The larvae were restrained with the aid of a thin layer of 2% low melting point agarose. The Clampfit version 10.6.2 software (Molecular Devices Corporation, USA) was used for processing the EEG recordings. The data were analysed manually by a trained observer, blind to the genotype of the larvae.