UV absorption titration was employed to study the binding interactions of the complexes with DNA in a UV-vis spectrophotometer (Cary 100) using reference Phosphate Buffer. The emission spectra were recorded in Fluorolog 3-111 (Horiba JobinYvon) spectrophotometer by noting the changes of fluorescence intensities of the ethidium bromide-DNA adduct with gradual addition of the complexes.
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