αdBigH1 antibodies are described in (6 (link)). αdH1 antibodies were a gift from Dr J. Kadonaga and are described in (16 (link)). αRpb3 antibodies were a gift from Dr J. Zeitlinger and are described in (17 (link)). The rest of antibodies used in these experiments were commercially available: αPol IIoser5 (Abcam, ab5131), αH3Kac (Millipore 06-599), αH3K36me3 (Cell Signaling, 9715), αH3 (Abcam, ab9050), αH4 (Abcam, ab10158) and αFLAG (Sigma F3165).
Recombinant His-tagged dBigH1 constructs were expressed in Escherichia coli BL21-LysS cells using pET30b(+) expression vectors (Novagen), and purified using Ni-NTA columns (BioRad) by conventional methods.
Recombinant His-tagged dBigH1 constructs were expressed in Escherichia coli BL21-LysS cells using pET30b(+) expression vectors (Novagen), and purified using Ni-NTA columns (BioRad) by conventional methods.
