Bone marrow cells from C57BL/6 mice were filtered through a 70-μm cell strainer in PBS and neutrophils were isolated by negative MACS selection (Miltenyi Biotec) as described previously (Hasenberg et al., 2011 (link)) using biotinylated anti-TER119 (clone TER119, 1 μg/ml), anti-CD5 (clone 53-7.3, 2.5 μg/ml), anti-CD45R (clone RA3-6B2, 1.25 μg/ml), anti-F4/80 (clone BM8, 5 μg/ml), anti-CD117 (clone 2B8, 1 μg/ml) and anti-CD49b (clone HMa2, 2.5 μg/ml) antibodies (all from Biolegend).
Isolation of neutrophils from C57BL/6 mouse bone marrow cells using negative MACS selection
dependent variables
Not explicitly mentioned
control variables
Use of C57BL/6 mouse bone marrow cells
Filtering of bone marrow cells through a 70-μm cell strainer in PBS
Use of biotinylated antibodies against TER119, CD5, CD45R, F4/80, CD117, and CD49b for negative MACS selection
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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