Formalin-fixed, paraffin-embedded livers were sectioned, and hematoxylin and eosin (H&E) stained slides were reviewed by two pathologists (K.A.M and S.C.) to determine histological features. Immunohistochemistry (IHC) was performed as previously described, using rabbit anti-cleaved Caspase 3 (Epitomics, Burlingame, CA) or rat anti-Ly6G (Biolegend, San Diego, CA) primary antibodies (25 (link)) to determine the extent of apoptosis and neutrophilic infiltration, respectively. Briefly, sections (5μm) were deparaffinized, rehydrated in alcohols, and subjected to heat-induced epitope retrieval in 0.1M citrate buffer at pH 6.0 in a microwave for 20 minutes. Slides were then incubated with anti-cleaved Caspase 3 (1:1000) or anti-Ly6G (1:1000) antibody for 1 hour at room temperature. After incubation with secondary antibody, a subsequent reaction was performed with biotin-free horseradish peroxidase enzyme-labeled polymer, 3,3′-diaminobenzidine was used as the chromogen, and sections were counterstained with hematoxylin.