Ethanol-Induced Liver Injury Model in Mice

All animal experiments were approved by the Institutional Animal Care and Use Committee (Approval No. KA2018-18) of the Korea Advanced Institute of Science and Technology (KAIST, Republic of Korea). Mice were maintained on a standard 12-h light-dark cycle in a specific-pathogen-free facility at KAIST. Wild-type male mice aged 8 to 10 weeks with a C57BL/6J background were fed a Lieber-DeCarli ethanol diet (#710260, Dyets Inc.) containing isocaloric maltose-dextrin (Pair) or 5% EtOH for 2 to 8 weeks. C57BL/6J background Adrb1/2 double knockout (DKO) and Alb-Cre mice were purchased from Jackson Laboratories (Stock Nos. 003810 and 003574, respectively). C57BL/6N background Gdf15^f/f and Cyp2e1^f/f mice were purchased from the EMMA mouse repository (Stock Nos. EM10460 and EM06364, respectively). Flippase mice were provided by the Korea Research Institute of Bioscience and Biotechnology Laboratory Animal Resource Center (Stock No. PX00003369). In all experiments with genetically modified mice, littermates were used as controls. Body weight changes and diet intake were measured daily. For pharmacological inhibition of monoamine oxidases, mice were treated with selegiline (Sigma‒Aldrich; 10 mg kg⁻¹ body weight, i.p., every other day) for the last 2 weeks of the experiments. All interventions were performed during the light cycle.