C. gloeosporioides isolate, obtained from citrus fruit showing typical symptoms of disease and previously identified, belongs to Di3A collection (Dipartimento di Agricoltura Alimentazione e Ambiente, University of Catania, Italy) [16 (link)]. The isolate, kept in Potato Dextrose Agar (PDA, Oxoid, Basingstoke Hempshire, UK) at 4 °C, was used in the in vitro and in growth chamber assays. Subcultures were grown for 10 days at 25 °C in the dark before each assay.
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