Antimicrobial susceptibility assays were performed according to a method described in our recent studies [9 (link),10 (link)]. Blank disks (6 mm, Oxoid, Basingstoke, UK) and Mueller–Hinton (MH) were purchased from Oxoid, Basingstoke, UK. An aliquot of 10 μL crude extract (500 μg/mL) was added onto each disk and the bacteriostatic effect on the corresponding strains evaluated by measuring the diameter of the inhibition zone after incubation at 37 °C for 12 h. A gentamicin disk (10 μg, Oxoid, Basingstoke, UK) was used as a positive control, while the methanol phase with water and chloroform phase with anhydrous ethanol were used as negative controls.
Broth dilution testing (microdilution) was carried out to determine MICs of the extracts according to the standard method issued by the Clinical and Laboratory Standards Institute, USA (CLSI, M100-S28, 2018). The standard solution of gentamicin (100 µg/mL) was purchased from the National Standard Material Information Center, Beijing, China [9 (link)].
Broth dilution testing (microdilution) was carried out to determine MICs of the extracts according to the standard method issued by the Clinical and Laboratory Standards Institute, USA (CLSI, M100-S28, 2018). The standard solution of gentamicin (100 µg/mL) was purchased from the National Standard Material Information Center, Beijing, China [9 (link)].
Free full text: Click here
