Controlled ovarian hyperstimulation (COH) was conducted with human chorionic gonadotropin (hCG). Gonadotropin-releasing hormone (GnRH) antagonists constitute a multiple-dose flexible regimen for the prevention of ovarian hyperstimulation syndrome (OHSS). Intracytoplasmic sperm injection (ICSI) was performed on metaphase II (MII) oocytes, and the resultant embryos were cultured to the blastocyst stage for biopsy. The biopsied blastocysts were cryopreserved for further embryo transfer cycles utilizing the vitrification procedure with individual tubes containing single blastocysts.
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