Monitoring the biosynthesis of secondary metabolites in agar-based fungal cultures in situ was performed using the droplet probe coupled with a Waters Acquity ultra-performance liquid chromatography (UPLC) system (Waters Corporation) to a Thermo QExactive Plus (Thermo Fisher Scientific) via procedures described previously [16 (link),26 (link)–29 ] and reviewed recently [16 (link)]. This technique probes the chemistry of the cultures via a mini-extraction of about 2 μL of 1:1 MeOH:H2O, permitting the mapping of secondary metabolites in live fungal cultures [26 (link)–28 (link),41 (link)].
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In situ fungal metabolite mapping
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Other organizations : University of North Carolina at Greensboro, Universidad Nacional Autónoma de México
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Variable analysis
independent variables
- None explicitly mentioned
- Biosynthesis of secondary metabolites in agar-based fungal cultures
- None explicitly mentioned
- No positive or negative controls were specified by the authors.
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