Tethered Ligand Induced Signaling in HEK293 Cells
Corresponding Organization :
Other organizations : Tufts Medical Center, Tufts University
Variable analysis
- Soluble and lipidated peptides
- Tethered ligands
- Amidated endogenous ligands: s-SubP-NH2, s-CCK4-NH2, and sulfated s-CCK8-NH2
- Antagonists: CP 99994 and YM022
- Tethered ligand induced signaling
- Activity of experimental ligands (MTLs and SMALs)
- Activity of receptors with amidated endogenous ligands
- Luciferase and β-galactosidase activities
- HEK293 cells
- Transfection time (24 hours for tethered ligands, 20 hours for soluble and lipidated peptides)
- Serum-free medium for stimulation
- Stimulation duration (4 hours)
- Amidated endogenous ligands: s-SubP-NH2 for NK1-3R, s-CCK4-NH2 for CCK2R, and sulfated s-CCK8-NH2 for CCK1R
- None specified
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