Bim-specific and mock small interfering RNAs (siRNAs) were purchased from Dharmacon RNA Tech (Lafayette, CO). Transfection of anti-Bim or the control siRNA duplexes into EOL-1 cells involved use of the Cell Line Nucleofector Kit T (Amaxa, Gaithersburg, MD) and program O-17 according to the manufacturer's instructions [23 (link),24 (link)]. Twenty-four h after siRNA transfection, the cells were exposed to drug treatment.
Free full text: Click here