Pollen Viability Assessment using Acetocarmine

Acetocarmine dye was used to investigate pollen viability. Ripe pollen grains from newly opened anthers were transferred onto a clean slide, and a few drops of aceto-carmine were added to the slide. After 20 minutes, stained pollen grains were considered fertile (viable), and the unstained pollen grains sterile (non-living). A total of 4000 pollen grains were counted, and the percentage of viability was calculated.
All observations and photography were carried out using an Olympus CX21 light microscope and the KAMERAM software program (Argenit, Türkiye).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kartal Ç. (2024). Reproductive biology of Centaurea kilaea (Asteraceae, Cardueae) – an endemic species from Türkiye. Acta Botanica Croatica, 84(1).

Publication 2024

Corresponding Organization : Trakya University

Top 5 similar protocols

Variable analysis

independent variables

Acetocarmine dye

dependent variables

Pollen viability

control variables

Newly opened anthers
Clean slide
20 minutes of staining time

controls

Stained pollen grains were considered fertile (viable)
Unstained pollen grains were considered sterile (non-living)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!